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Heterologous expression of signal protein 14-3-3 in and the subsequent immune response in mice

ZHENG Meijuan, SHEN Jilong, LUO Qingli, XU Yuanhong

《医学前沿（英文）》 2008年第2卷第1期页码 95-99 doi: 10.1007/s11684-008-0017-y

摘要： Schistosomiasis japonica, a zoonosis caused by , is endemic to the Philippines and China. Several vaccine candidates have been identified and tested in different animal models, but it is still unclear which will be optimal for testing in the field. Therefore, new antigens and strategies are necessary for vaccine development against schistosomiasis japonica. The Sj14-3-3 gene was amplified and subcloned into the expression vector pPICZ?-B and transformed into X-33 by electroporation. Three transformants were induced with methanol. The cultural supernatant was collected and tested by SDS-PAGE and Western blotting. The protein of rSj14-3-3 was prepared and purified and BALB/c mice were immunized which was followed by a challenging infection. The immuno-protection was then evaluated. The Sj14-3-3 gene was expressed and secreted into the medium and its molecular weight was about 35000 as determined by SDS-PAGE. Western blotting showed that the protein had a high specificity against mouse-anti-Sj14-3-3 monoclonal antibody and rSj14-3-3 had a promising immune reactivity. The results of the immuno-protective experiments revealed that the worm reduction was 26.0%, 32.2%, and 36.8%, respectively. The number of eggs in liver tissue was reduced by 36.8%, 43.2%, and 46.1%, respectively. The recombinant Sj14-3-3 of eukaryotic expression in was successfully harvested. The molecular vaccine of Sj14-3-3 could partially induce resistance to the infection with in BALB/c mice. The recombinant protein Sj14-3-3 has promising immunological potentials for further approach to the diagnosis and development of molecular vaccine.

关键词： development challenging rSj14-3-3 resistance cultural supernatant

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Implement and Innovate Environmental Protection Measures, Promote Green Protection of Cultural Relics

Fu-qing Liang,Yong-ping Sun

《工程管理前沿（英文）》 2014年第1卷第4期页码 330-335 doi: 10.15302/J-FEM-2014055

摘要： The subject is implementing and innovating environmental protection measures, and promoting green protection of cultural relics in the Three Gorges area. This thesis states its significance and effect in the beginning, and then summarizes seven pieces of main practice, includes five pieces of main effect. In the end, this thesis produces some relevant conclusions and suggestions about the subject.

关键词： environmental science the Three Gorges cultural relics innovate environmental protection measures green protection

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Cultural differences define diagnosis and genomic medicine practice: implications for undiagnosed diseases

null

《医学前沿（英文）》 2013年第7卷第3期页码 389-394 doi: 10.1007/s11684-013-0281-3

摘要：

Despite the current acceleration and increasing leadership of Chinese genetics research, genetics and its clinical application have largely been imported to China from the Occident. Neither genetics nor the scientific reductionism underpinning its clinical application is integral to the traditional Chinese worldview. Given that disease concepts and their incumbent diagnoses are historically derived and culturally meaningful, we hypothesize that the cultural expectations of genetic diagnoses and medical genetics practice differ between the Occident and China. Specifically, we suggest that an undiagnosed diseases program in China will differ from the recently established Undiagnosed Diseases Program at the United States National Institutes of Health; a culturally sensitive concept will integrate traditional Chinese understanding of disease with the scientific reductionism of Occidental medicine.

关键词： genetics cultural differences undiagnosed diseases program

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“一带一路”工程科技人文交流的基本内涵与企业参与现状

姚威 ,胡顺顺 ,牛丰

《中国工程科学》 2019年第21卷第4期页码 111-117 doi: 10.15302/J-SSCAE-2019.04.017

摘要：

工程科技人文交流是人类改造或创造事物过程中不同科技知识和价值观等思想内容的交流过程，其目的是减少或消除不同国家间工程科技思想隔阂，增进民心相通，确保工程项目的顺利实施。本文从工程科技人文交流的内涵以及企业参与工程科技人文交流的重要意义出发，研究“一带一路”重点建设企业的工程科技人文交流现状，剖析企业参与工程科技人文交流存在的问题，进而对我国企业深度参与工程科技人文交流提出初步的建议。

关键词： “一带一路” 人文交流工程科技企业

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秦巴文化旅游产业发展战略研究

张凡,胡永红,段德罡

《中国工程科学》 2016年第18卷第5期页码 46-51 doi: 10.15302/J-SSCAE-2016.05.007

摘要：

秦巴山区既是世界级名山和华夏文明发源地，同时也是我国重要生态环境保护区和连片贫困地区之一，区内面临着经济发展与生态保护的矛盾。本文结合秦巴山区的文化旅游资源特点，顺应旅游产业发展趋势，提出了发展文化旅游产业、推动秦巴山区突破发展的基本思路。在分析秦巴文化旅游资源价值的基础上，文章明确了秦巴文化旅游产业发展的战略思路、战略目标、战略重点以及空间布局，并提出了秦巴文化旅游产业发展的对策建议。

关键词：秦巴山区文化旅游产业发展战略

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Co-application of energy uncoupling and ultrafiltration in sludge treatment: Evaluations of sludge reduction, supernatant

An Ding, Yingxue Zhao, Zhongsen Yan, Langming Bai, Haiyang Yang, Heng Liang, Guibai Li, Nanqi Ren

《环境科学与工程前沿（英文）》 2020年第14卷第4期 doi: 10.1007/s11783-020-1238-9

摘要： Effects of metabolic uncouplers addition on sludge reduction were carried out. TCS addition effectively inhibited ATP synthesis and reduced sludge yield. The effluent quality such as TOC and ammonia deteriorated but not significantly. Suitable dosage retarded biofouling during sludge water recovery by UF membrane. Energy uncoupling is often used for sludge reduction because it is easy to operate and does not require a significant amount of extra equipments (i.e. no additional tank required). However, over time the supernatant extracted using this method can deteriorate, ultimately requiring further treatment. The purpose of this study was to determine the effect of using a low-pressure ultrafiltration membrane process for sludge water recovery after the sludge had undergone an energy uncoupling treatment (using 3,3′,4′,5-tetrachlorosalicylanilide (TCS)). Energy uncoupling was found to break apart sludge floc by reducing extracellular polymeric substances (EPS) and adenosine triphosphate (ATP) content. Analysis of supernatant indicated that when energy uncoupling and membrane filtration were co-applied and the TCS dosage was below 30 mg/L, there was no significant deterioration in organic component removal. However, ammonia and phosphate concentrations were found to increase as the concentration of TCS added increased. Additionally, due to low sludge concentrations and EPS contents, addition of 30–60 mg/L TCS during sludge reduction increased the permeate flux (two times higher than the control) and decreased the hydraulic reversible and cake layer resistances. In contrast, high dosage of TCS aggravated membrane fouling by forming compact fouling layers. In general, this study found that the co-application of energy uncoupling and membrane filtration processes represents an effective alternative method for simultaneous sludge reduction and sludge supernatant recovery.

关键词： Sludge reduction Energy uncoupling Ultrafiltration membrane Membrane fouling

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公共文化建设项目应用公私伙伴关系模式的探讨

郭峰,蔡艺卿,王飞球

《中国工程科学》 2013年第15卷第11期页码 108-112

摘要：

为改善我国公共文化建设项目的效率和提高公共文化服务水平，分析了公私伙伴关系（PPP）模式在公共文化建设项目的应用。本文研究对象是PPP模式在我国的应用现状和公共文化建设项目的特殊性，分析了PPP 模式在我国公共文化建设项目的适用性及优势，以宁波文化广场为例分析了PPP 模式的应用过程，并提出实现我国公共文化建筑PPP模式的几点建议。

关键词： PPP模式公共文化建设项目应用分析

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Studies of ultrasound disintegration of residual sludge and its energy consumption in water treatment of petrochemical plant

SHEN Jinfeng, YIN Xuan, GU Heping, Lv Xiaoping

《化学科学与工程前沿（英文）》 2007年第1卷第4期页码 395-398 doi: 10.1007/s11705-007-0072-y

摘要： To investigate the influence of ultrasound pretreatment on sludge anaerobic digestion, the ultrasound disintegration of residual sludge in water treatment of petrochemical plant was studied, and the mechanisms of ultrasound and medium were introduced. Experimental results indicate that ultrasound cavitation induces the rise of sludge temperature, which improves ultrasound disintegration on sludge. U1trasound pretreatment can advance observably the quantity of chemical oxygen demand in sludge supernatant fluid (SCOD), which increases with ultrasound intensity and sonication time. The degree of ultrasound disintegration increases with the specific energy input. When the specific energy input is l0 000 kJ/kg of total dry solids, the degree of ultrasonic sludge disintegration reaches 40%.

关键词： specific supernatant petrochemical ultrasound intensity temperature

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Effect of arsenic trioxide on proliferation and apoptosis of U266 cells and its relationship with the expression variation of VEGF

ZHAN Rong, YU Qinghong, HUANG Haobo

《医学前沿（英文）》 2008年第2卷第4期页码 356-360 doi: 10.1007/s11684-008-0068-0

摘要： The aim of this article is to explore the effect of arsenic trioxide (AsO) on the proliferation and apoptosis of myeloma cell line U266 and its relationship with the expression variation of vascular endothelial growth factor (VEGF). The viability and apoptosis of U266 cells were observed by methylthiazolyl- tetrazolium (MTT) assay and terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL). The effect of AsO on the VEGF expression of U266 cells were tested by enzyme linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. We found that AsO could significantly inhibit the growth of U266 cells, and the concentration for 50% growth inhibition (IC) was 2 ?mol/L. After treatment with 2, 5, 10 ?mol/L AsO for 36 hours, dose-dependent apoptosis of U266 cells was observed. After treatment with 2, 5, 10 ?mol/L AsO for 72 hours, a dose-dependent reduction of VEGF in the supernatant of U266 cells culture was found. As far as single cells are concerned, nevertheless, the expression of VEGF mRNA did not vary. So we draw the conclusion that AsO could induce the apoptosis of U266 cells and inhibit their proliferation, decrease the tumor load, and lead to the reduction of VEGF in the culture supernatant, but not change the expression of VEGF in single U266 cells.

关键词： VEGF expression expression variation culture supernatant labeling concentration

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基于文化传承的微介入乡村规划策略研究

郭海鞍

《中国工程科学》 2019年第21卷第2期页码 27-33 doi: 10.15302/J-SSCAE-2019.02.019

摘要：

长期以来，乡村规划一直沿袭城市规划设计的体系，以总图或鸟瞰图来表达对乡村规划的成果。很多地方政府一轮一轮做规划，但乡村风貌的改善效果甚微，这是因为忽视了乡村内在的文化传承问题，忽视了文化价值观的内在作用。源于城市的规划设计方法不能草率地划定乡村生活，因此需要研究一种与乡村文化传承相适应的规划设计理论。微介入规划理论是针对文化引导下的乡村复兴规划策略，基于一种小微渐进的方法，逐步实现乡村的改造与复兴，这种策略在当今大拆大改气势正盛的新一轮乡村规划进程中，显得尤为重要。

关键词：乡村规划微介入规划推演、容错乡村文化乡村复兴

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我国数字创意产业发展现状及创新方向

黄江杰,汤永川,孙守迁

《中国工程科学》 2020年第22卷第2期页码 55-62 doi: 10.15302/J-SSCAE-2020.02.008

摘要：

数字创意产业以科学技术和文化艺术为输入，以经济价值和文化影响为输出，是驾驭数字技术的创意内容业和创意制造业的总称，在“十三五”时期首次被列为国家战略性新兴产业，为我国产业转型升级、数字经济繁荣发展发挥了重要作用。本文旨在从宏观层面研究数字创意产业的创新发展方向，总结我国数字创意产业的发展经验并探讨产业未来发展建议。通过比较分析国内外数字创意产业的概念、内涵和发展思路，系统梳理了我国数字创意产业的发展特征、发展状况以及存在问题，并据此形成了我国数字创意产业的总体发展思路。研究提出了我国数字创意产业持续性创新和发展的四大方向：数字文化创意技术装备创新、数字文化内容创新、创新设计发展、融合渗透创新发展，并在数字创意产业的科技攻关、创新机构、平台企业、人才培养、监管服务、产业聚集等方面提出了对策建议。

关键词：数字创意产业技术装备文化内容创新设计融合发展

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我国数字创意技术的发展现状与展望

邓磊,王妙辉,范雷东,袁圆,王振中

《中国工程科学》 2020年第22卷第2期页码 63-70 doi: 10.15302/J-SSCAE-2020.02.009

摘要：

数字创意产业作为国家“十三五”规划确定的战略性新兴产业之一，以全球数字化和网络化为背景，以文化创意、内容生产、版权利用为发展核心，通过融合渗透带动周边产业集群的发展。相关产业发展壮大的基础性支撑在于数字创意技术与创新设计，而这又是我国产业发展的薄弱环节，且面临未来发展路径的缺失状态。本文结合产业数据调研结果，系统梳理了我国数字创意技术中的使能技术、应用技术和终端设备技术的发展现状，总结了超高清视频、三维声、虚拟现实 / 增强现实（VR/AR）、数字内容生产和创新设计软件等具体方向存在的问题与短板。研究表明，设立数字创意技术国家科技重大专项，建设数字创意技术国家工程研究中心，提高数字创意技术人才培养质量，积极参与国际合作并扩大中国文化的影响力，将有力推动我国数字创意技术的中长期发展。

关键词：数字创意文化创意创新设计超高清视频虚拟现实

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中国铁路运营“走出去”发展策略研究

高峰,刘东,李文杰,郑乃明,马思远

《中国工程科学》 2017年第19卷第5期页码 44-48 doi: 10.15302/J-SSCAE-2017.05.008

摘要：

本文深入分析了中国铁路运营“走出去”存在的问题，如中国铁路建设与运营脱节、运营企业缺乏“走出去”的主动性、跨文化管理经验不足等问题，提出了转化现有理念和机制，融合国内外标准、规章、维修、安全、能力、绩效评价等管理体系，实现运营角色由提供服务向提供咨询的转变，加强跨文化管理人才储备，完善人才培养与储备机制等发展策略。

关键词：中国铁路运营管理体系跨文化

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Marble epistyles under shear: an experimental study of the role of “Relieving Space”

E. D. PASIOU, I. STAVRAKAS, D. TRIANTIS, S. K. KOURKOULIS

《结构与土木工程前沿（英文）》 2019年第13卷第4期页码 767-786 doi: 10.1007/s11709-019-0515-9

摘要： The mechanical response of mutually interconnected epistyles is studied experimentally. The specimens are made of two marble blocks connected to each other with an “I”-shaped titanium connector placed in grooves sculptured on the blocks and covered with cementitious material. The specific way of connecting epistyles simulates the one used by scientists restoring the ancient “connections” of the epistyles of the Parthenon Temple. This “connection,” although designed to sustain mainly tensile loading, undertakes, also, shear load, in case of excitations imposing to the epistyles displacements normal to the connector’s axis. Attention is focused to enlighten the role of a novel design technique aiming to relieve the stress field around the connector. According to this technique, part of the connector’s web is left uncovered, forming the so-called “ ,” assisting the unconstrained deformation of the connector. Both traditional and innovative sensing techniques were employed in an effort to obtain data from the interior of the three-material-complex (marble-cementitious material-titanium). Analysis of the data indicated that the “Relieving Space” reduces the overall stiffness of the system, protecting marble in case of over-loading. Moreover, it was concluded that the innovative techniques employed provide pre-failure indicators well in advance of the catastrophic failure of the specimens.

关键词： monuments of cultural heritage marble epistyles pressure stimulated currents acoustic emission digital image correlation